Antioxidant Activity And Hepatoprotective Property Of Breadnut Seed (Artocarpus Camansi Blanco) Extract Aganinst Carbon Tetrachloride-Induced Liver Damage In Albino Rats / Ayra Daniele L. Alberto., Czar Ianne L. Idor., Jamaica F. Mangabat., Julius M. Vitin
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TextPublication details: 2014Description: ix, 76 leaves 28 cmSummary: ABSTRACT ALBERTO, ADL., IDOR, CIL., MANGABAT, JF., VITIN, JM. Mariano Marcos State University, College of Health Sciences, Department of Pharmacy, MARCH 2014. “Antioxidant Activity And Hepatoprotective Property Of Breadnut Seed (Artocarpus Camansi Blanco) Extract Aganinst Carbon Tetrachloride-Induced Liver Damage In Albino Rats.”
Adviser: Ms. Ms. Angelica May A. dela Cruz
This experimental study was conducted to investigate the antioxidant activity and hepatoprotective property of Breadnut seed (Artocarpus camansi Blanco) extract against carbon tetrachloride-induced liver damage in albino rats. Artocarpus camansi Blanco extract was air-dried for four days and oven-dried for two days at 40°C then milled. Plant extract was obtained by 72-hour maceration with ethanol as solvent and rotary evaporation methods.
The antioxidant property of ethanolic extracts of breadnut seed was evaluated by DPPH assay and TPC (total phenolic content) by Folin-Ciocalteau method. DPPH (1, 1-diphenyl-2-picrylhydrazil) assay of ACBE showed 11.427% free radical inhibition at a dose of mcg/ml. total phenolic content was determined by spectrophotometry using gallic acid as the standard. The obtained total polyphenols as GAE per milligram extract was 33.208 mcg.
For the hepatoprotective activity, test animals were divided into 4 groups with 3 animals each. For 7 days, group 1 (positive control), received Silymarin (25mg/kg), group 2 (negative control), received PNSS (10ml/kg); group 3 (treated group), received ACBE (0.2g/kg,po). By administering Carbon tetrachloride, the destruction in the liver was observed. Through tain vein puncture of the blood, changes in levels of biochemical markers of hepatic damage like SGOT and SGPT with histopathologic examination of the liver were tested in all groups. Treatments with ACBE (320mcg/ml) showed minimal efficacy. ACBE and positive control Silymarin showed no significant difference.
The significant differences in the treatments were analyzed by t-test between results. There is a significant difference between Ascorbic acid and ACBE while the hepatoprotective activity between Silymarin and ACBE showed no significant difference.
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Thesis (Bachelor of Science in Pharmacy) -- Mariano Marcos State University - College of Health Sciences, Batac City
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ABSTRACT ALBERTO, ADL., IDOR, CIL., MANGABAT, JF., VITIN, JM. Mariano Marcos State University, College of Health Sciences, Department of Pharmacy, MARCH 2014. “Antioxidant Activity And Hepatoprotective Property Of Breadnut Seed (Artocarpus Camansi Blanco) Extract Aganinst Carbon Tetrachloride-Induced Liver Damage In Albino Rats.”
Adviser: Ms. Ms. Angelica May A. dela Cruz
This experimental study was conducted to investigate the antioxidant activity and hepatoprotective property of Breadnut seed (Artocarpus camansi Blanco) extract against carbon tetrachloride-induced liver damage in albino rats. Artocarpus camansi Blanco extract was air-dried for four days and oven-dried for two days at 40°C then milled. Plant extract was obtained by 72-hour maceration with ethanol as solvent and rotary evaporation methods.
The antioxidant property of ethanolic extracts of breadnut seed was evaluated by DPPH assay and TPC (total phenolic content) by Folin-Ciocalteau method. DPPH (1, 1-diphenyl-2-picrylhydrazil) assay of ACBE showed 11.427% free radical inhibition at a dose of mcg/ml. total phenolic content was determined by spectrophotometry using gallic acid as the standard. The obtained total polyphenols as GAE per milligram extract was 33.208 mcg.
For the hepatoprotective activity, test animals were divided into 4 groups with 3 animals each. For 7 days, group 1 (positive control), received Silymarin (25mg/kg), group 2 (negative control), received PNSS (10ml/kg); group 3 (treated group), received ACBE (0.2g/kg,po). By administering Carbon tetrachloride, the destruction in the liver was observed. Through tain vein puncture of the blood, changes in levels of biochemical markers of hepatic damage like SGOT and SGPT with histopathologic examination of the liver were tested in all groups. Treatments with ACBE (320mcg/ml) showed minimal efficacy. ACBE and positive control Silymarin showed no significant difference.
The significant differences in the treatments were analyzed by t-test between results. There is a significant difference between Ascorbic acid and ACBE while the hepatoprotective activity between Silymarin and ACBE showed no significant difference.
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